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发布于:2019-6-27 09:21:05  访问:6 次 回复:0 篇
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V-preS2/S induced by straight injection of naked plasmids containing variable
Subsequent studies by Test et al.[3] showed that covalent conjugates of C3d and the capsular polysaccharide of serotype 14 streptococcus pneumoniae (PPS14) elicited higher titers to PPS14 in mice than PPS14 only, and furthermore induced a class switch FluazinamMedChemExpress 24249315" title=View Abstract(s)">PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/24249315 in anti-PPS14 from predominantly IgM to IgG1, which extended the adjuvant effects of C3d to T-independent AstaxanthinIn Vitro antigen as well as T-dependent antigen. BALB/c mice have been primed by intramuscular gene immunization with one hundred various recombinant plasmids on day 0 and were boosted by subcutaneous inoculation with HBsAg protein (1 ) 12 wk post-priming. The levels and avidity of particular IgG in sera collected at the indicated occasions from every single group have been determined by ELISA and NaSCN-displacement ELISA, respectively. Final results: HBsAg specific antibody response was elicited in groups primed with plasmids pVAON33-S2/S-P28.[1-4] and pVAON33-S2/S. Nevertheless, the response against HBsAg within the groups primed with pVAON33-S2/S-P28.[1-4] was significantly higher than that in pVAON33-S2/S group, the highest degree of the certain antibody response was observed in the groups primed with pVAON33-S2/S-P28.4 (P<0.01). Outcomes: HBsAg specific antibody response was elicited in groups primed with plasmids pVAON33-S2/S-P28.[1-4] and pVAON33-S2/S. Nonetheless, the response against HBsAg inside the groups primed with pVAON33-S2/S-P28.[1-4] was substantially higher than that in pVAON33-S2/S group, the highest level of the particular antibody response was observed inside the groups primed with pVAON33-S2/S-P28.4 (P<0.01). After secondary immunization with specific antigen, the acceleration of antibody levels was significantly higher and faster in the mice primed with DNA expressing preS2/S-P28 fusions than that with DNA expressing preS2/S only (P<0.05). Interestingly, mice primed with DNA expressing preS2/SP28.4 fusions maintained the highest levels of anti-HBs antibodies in all animals. The avidity assay showed that the avidity index (AI) collected at 18 wk from mice primed with pVAON33-S2/S-P28.3 and pVAON33-S2/S-P28.4 were significantly higher than that from preS2/S-DNA vaccinated mice (P<0.01).INTRODUCTION The third complement protein (C3) plays a major role in the complement activation pathway, the critical role of the cleavage fragments of C3 in the humoral immune response for both Tdependent and T-independent antigens was reported in studies performed over a quarter of a century ago[1,2]. Complement‘s potential use as an adjuvant in vaccines was first suggested when Dempsey et al.[2] demonstrated that mice, vaccinated with a genetically engineered construct containing three copies of mouse C3d fused to a model antigen, hen egg lysozyme, increased the efficiency of immunizations by more than 1 000fold. Subsequent studies by Test et al.[3] showed that covalent conjugates of C3d and the capsular polysaccharide of serotype 14 streptococcus pneumoniae (PPS14) elicited higher titers to PPS14 in mice than PPS14 only, and furthermore induced a class switch PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/24249315 in anti-PPS14 from predominantly IgM to IgG1, which extended the adjuvant effects of C3d to T-independent antigen as well as T-dependent antigen.
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